Abstract
A liquid chromatographic method is described for the simultaneous determination of retinoic acid, retinol, and retinyl palmitate in ram plasma. Samples of 0.2 mL are extracted with 2‐propanol‐dichloromethane, the extracts are centrifuged, the supernatants are collected, evaporated, reconstituted in mobile phase, and analyzed on a C8 column using two consecutive isocratic elutions with methanol tetrahydrofuran‐acetate buffer. Detection is performed at 350 and 325 nm using wavelength change during the run. The method exhibits analytical characteristics well within acceptable limits. Overall recoveries were 73.7% for retinoic acid, 90.2% for retinol, and 87.7% for retinyl palmitate. Precision values, expressed as % relative standard deviation, were in the range of 1.16–6.18%, while limits of detection were in the range of 0.3–10.0 ng/mL for all analytes.
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