SIMULTANEOUS DETERMINATION OF PREDNISOLONE ACETATE AND MOXIFLOXACIN HYDROCHLORIDE IN BULK AND IN EYE DROP USING RP–HPTLC

Abstract

A simple, rapid, and precise RP-HPTLC method for analysis of Prednisolone acetate and Moxifloxacin hydrochloride in their eye drop dosage forms has been developed and validated. Chromatography was performed on silica gel 60 RP-18F254S plates with Methanol:Water:Triethylamine 7:3:0.3 (v/v) as mobile phase. Detection was performed at 274 nm. The Rf values for Prednisolone acetate and Moxifloxacin hydrochloride were found to be 0.50 ± 0.02 and 0.72 ± 0.02, respectively. Regression plots revealed good linear relationships in the concentration ranges 600–3600 ng/band for Prednisolone acetate and 300–1800 ng/band for Moxifloxacin hydrochloride. When accuracy was checked by conducting recovery studies the % recoveries were ranging from 100.97% to 102.03% (Prednisolone acetate) and 100.49% to 101.25% (Moxifloxacin hydrochloride), respectively. The amount of Prednisolone acetate and Moxifloxacin hydrochloride estimated in marketed formulation were 99.22% and 101.07%, respectively.