Abstract
To establish a RP-HPLC method for simultaneous determination of phenylethanoid glycosides plantainoside D and verbascoside in Chirita longgangensis var. hongyao. The analysis was performed on a Agilent C18 column (4.6 mm x 250 mm, 5 microm) with CH3CN-1% HAc (16:84)as mobile phase at a flow rate of 1.0 mL x min(-1), and at a column temperature of 30 degrees C. The detection wave length was 332 nm. The linear ranges of calibration of plantainoside D and verbascoside were 3.125-100.00 mg x L(-1) (r = 0.9998) and 25.00-500.0 mg x L(-1) (r = 0.9998). The average recoveries were 101.3% and 100.8% with RSD of 2.6% and 2.2% (n=9), respectively. The method is simple, accurate, reliable and can be used for the quality evaluation of C. longgangensis var. hongyao and its preparation.
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