Abstract
The conditions for the simultaneous separation of aliphatic and aromatic amines, nitrites and nitrates by ion-interaction reversed-phase high-performance liquid chromatography (HPLC) were optimized. The mechanism that governs retention in this HPLC technique is discussed. Sensitivity of the order of 0.10 ppm for nitrites and nitrates can be obtained with octylamine orthophosphate as eluent at 210 nm. Amounts of 0.20 ppm of nitrates and 0.50 ppm of 1,4-phenylenediamine can be easily identified and determined in matrices as complex as sea water.
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