Abstract
A simple and sensitive HPLC-DAD detection method was established for the simultaneous determination of nine compounds including oxypaeoniflorin, albiflorin, paeoniflorin, benzoylpaeoniflorin, glycyrrhizic acid, liquiritin, isoliquiritin, liquiritigenin, and isoliquiritigenin in the Traditional Chinese Medicinal Prescription Shaoyao-Gancao-Tang (SGT) and we analyze the relationship between therapeutical effect and compatibility of medicines by using an Agilent extend-C18 column at a flow rate of 1 mL/min. The column temperature was maintained at 30°C and the detection wavelength was set at 230 nm for oxypaeoniflorin, albiflorin, paeoniflorin, benzoylpaeoniflorin, and glycyrrhizic acid; 276 nm for liquiritin and liquiritigenin; 360 nm for isoliquiritin and isoliquiritigenin. The total contents of the nine compounds in SGT varied from 4.65 to 20.06 mg/mL. The results of this study showed that the content of chemical compounds of Traditional Chinese Medicinal Prescription is mainly influenced by the dosage and compatibility of medicines and the therapeutical effect of Traditional Chinese Medicinal prescription is mainly influenced by the dosage and compatibility of medicines. The method could be suitable for quality control of SGT with bioactive multicompounds.
Highlights
SGT is a Traditional Chinese Prescription containing Radix Paeoniae alba and Radix Glycyrrhizae with the ratio of 1 : 1 and is commonly used to relieve spasm and pain
Oxypaeoniflorin, albiflorin, paeoniflorin, liquiritin, isoliquiritin, liquiritigenin, benzoylpaeoniflorin, isoliquiritigenin, and glycyrrhizic acid (Figure 1) are the most important bioactive constituents reported at present
The reference compounds of oxypaeoniflorin, albiflorin, paeoniflorin, liquiritin, isoliquiritin, liquiritigenin, benzoylpaeoniflorin, isoliquiritigenin, and glycyrrhizic acid were purchased from Chengdu Pufeide Biotech Co., Ltd. of China (Chengdu, China)
Summary
SGT is a Traditional Chinese Prescription containing Radix Paeoniae alba (stir-baked with vinegar) and Radix Glycyrrhizae (stir-baked with honey) with the ratio of 1 : 1 and is commonly used to relieve spasm and pain. Oxypaeoniflorin, albiflorin, paeoniflorin, liquiritin, isoliquiritin, liquiritigenin, benzoylpaeoniflorin, isoliquiritigenin, and glycyrrhizic acid (Figure 1) are the most important bioactive constituents reported at present. Liquiritigenin and glycyrrhizic acid inhibit liver cell injury and were given intravenously for the treatment of chronic viral hepatitis and cirrhosis in Japan [8, 9]. It has proven itself effective in the treatment of autoimmune hepatitis in one clinical trial [10]. Isoliquiritin and liquiritin have the Evidence-Based Complementary and Alternative Medicine
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
