Abstract
A simple, rapid, sensitive and selective method for the simultaneous determination of the non-steroidal anti-inflammatory drug naproxen (NAP) and its main metabolite the 6-demethylated derivative (DNAP) in human serum is described. The method is based on the intrinsic fluorescence of both acids in alkaline aqueous solution. The broad-band overlapping conventional spectra of both compounds are resolved by means of second-derivative synchronous fluorescence spectrometry, thus obviating the need for pre-analysis sample separation techniques. NAP and DNAP are determined in serum supernatant solution after removal of proteins with acetonitrile at pH 11.7. Recoveries from sera spiked with NAP (5–70 μg ml −1) and DNAP (0.25–5 μg ml −1) ranged from 95 to 106% (mean 99.6%) and from 95 to 104% (mean 100.1%), respectively.
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