Abstract
Lactic, acetic, propionic, isobutyric, isovaleric and valeric acids were simultaneously determined by gas-liquid chromatography. Lactic acid was oxidized to acetaldehyde by periodic acid and acetaldehyde was eluted before acetic acid. Separation of all acids via a column packed with 80/100 Chromosorb WAW, having GP 10% SP-1200/1% H 3PO 4 as the liquid phase, was complete in less than 5 min. The method was applied successfully to extracts of fermented corn plant material. Recoveriess of lactic, acetic and butyric acids were 93.1 ± 3.6, 105.1 ± 7.7 and 93.6 ± 3.2%, respectively, and the detection limits were equal to or less than 10, 1 and 0.5 nmol per injection.
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