Simultaneous determination of gallic acid，chlorogenic acid and caffeic acid contents in Ganmao’an granules by HPLC method

Abstract

Objective: To establish a HPLC method for simultaneous determination of gallic acid,chlorogenic acid and caffeic acid contents in Ganmao'an granules.Methods: The separation column of Kromasil C18(150 mm×4.6 mm,5 μm) was used,acetonitrile(A)-0.4% phosphoric acid(B) was applied as the mobile phase with gradient elution: 0-7.50 min 2% A,7.50-7.51 min 2%→12% A,7.51-25.00 min 12% A.The flow rate was 0.8 ml/min.The column temperature was room temperature.The detection wavelengths were 272 nm for gallic acid,327 nm for chlorogenic acid and caffeic acid.Results: The linear ranges of gallic acid,chlorogenic acid and caffeic acid were 0.27-8.64 μg/ml(r=0.999 9),1.00-32.00 μg/ml(r=0.999 7) and 0.30-9.60 μg/ml(r=0.999 8),respectively.The average recoveries of gallic acid,chlorogenic acid and caffeic acid were 100.14%,RSD 1.66%(n=9),98.10%,RSD 2.38%(n=9) and 100.48%,RSD 2.28%(n=9),respectively.Conclusion: The method is accurate,sensitive,selective and reproducible,and could provide the reference for the improvement of quality control standard of Ganmao'an granules.