Abstract
This study concerned the development and validation of a green and rapid method for the analysis of 23 pesticides in tobacco using ultra performance convergence chromatography-tandem mass spectrometry (UPC2-MS/MS) combined with modified QuEChERS. The sample was extracted with acetonitrile, purified by graphene, and then determined by UPC2-MS/MS. In this paper, optimization was made on factors affecting separation efficiency including chromatographic column, modifier, column temperature, and back pressure. With CO2-ethanol as mobile phase, all pesticides were adequately separated on a Viridis HSS C18 SB column, and the whole analysis process was completed within 6 min. Under optimal conditions, the calibration curves showed good linearity in the range of 10–200 ng/mL with the regression coefficients higher than 0.9946. The limit of detection and limit of quantification were 1.04–7.07 and 3.32–23.16 μg/kg, respectively. At three spiked levels (0.2, 1.0 and 2.0 mg/kg), mean recoveries for 23 pesticides were ranging from 80.2% to 110.6% and relative standard deviations were 2.2%–7.2%. The proposed method, being fast, sensitive, and practical, can be used for rapid detection of fungicides and carbamates in tobacco.
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