Abstract
We describe a new, rapid, and sensitive HPLC method with fluorescent detection for simultaneous analysis of plasma and urine free catecholamines, metanephrines, and polyamines without prior treatment procedures via precolumn derivatization with Fmoc-Cl. The separation gradient was 40 minutes. The results showed good linearity across the calibration range 0.05–20 µM and linearity coefficient (r 2) higher than 0.990, except for epinephrine, r 2 = 0.985. The LOQ was in range 680–1300 fmol and LOD 204–390 fmol of these analytes per injection 20 microlitres. The reproducibility of the method for the analyzed samples, expressed as R.S.D. was from 1.2 to 4.5%. Isoproterenol was used as internal standard.
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