Abstract
Lobelia chinensis Lour. (L. chinensis) has traditionally been used as a treatment for snake bites, high fever, jaundice, edema, and diarrhea, and modern studies have reported its anti-inflammatory, antioxidant, and antiviral activities. L. chinensis contains various compounds, such as flavonoids and coumarins, and its flavonoid components have been identified in many studies. In this study, a high-performance liquid chromatograph equipped with a photodiode array (PDA) detector and an Aegispak C18-L reverse-phase column (4.6 mm × 250 mm i.d., 5 μm) was used to simultaneously analyze four marker components in L. chinensis for standardization purposes. HPLC-PDA (detection at 340 nm), performed using a 0.1% formic acid-water/0.1% formic acid-acetonitrile gradient, separated the four marker compounds: luteolin-7-O-β-d-glucuronopyranosyl (1→2)-O-β-d-glucuronopyranoside, clerodendrin, chrysoeriol-7-O-diglucuronide, and diosmin. The developed analytical method showed excellent linearity values (r2 > 0.9991), limits of detection (LODs: 0.376–2.152 μg/mL), limits of quantification (LOQs: 1.147–6.521 μg/mL), intra- and inter-day precisions (RSD < 1.96%), and analyte recoveries (96.83–127.07%; RSD < 1.73%); thus, it was found to be suitable for the simultaneous analysis of these four marker compounds in L. chinensis.
Highlights
Lobelia chinensis Lour. (L. chinensis), a perennial herb of the Campanulaceae family, is distributed mainly in Indochina, Taiwan, Japan, China, and Korea
Using high-performance liquid chromatography combined with a diode-array detector and coupled with electrospray ionization with ion-trap time-of-flight mass spectrometry (HPLC-DAD-ESI-IT-TOF-MS), 11 compounds were identified from L. chinensis by comparing their retention times and MS spectra with those of standards or literature data [32,33]
Specificities were determined to confirm that L7GC, CDR, C7dGlu, and DSM were selectively separated from other compounds in the crude extract and determined using the retention times and absorbance wavelengths of the chromatograms obtained by analyzing samples and standard solutions with HPLC-photodiode array (PDA)
Summary
Lobelia chinensis Lour. (L. chinensis), a perennial herb of the Campanulaceae family, is distributed mainly in Indochina, Taiwan, Japan, China, and Korea. (L. chinensis), a perennial herb of the Campanulaceae family, is distributed mainly in Indochina, Taiwan, Japan, China, and Korea. Of the compounds in L. chinensis, diosmin, diosmetin, linarin, lobetyolinin, and lobetyolin are considered to be the main active components [8,12,13]. Using high-performance liquid chromatography combined with a diode-array detector and coupled with electrospray ionization with ion-trap time-of-flight mass spectrometry (HPLC-DAD-ESI-IT-TOF-MS), 11 compounds were identified from L. chinensis by comparing their retention times and MS spectra with those of standards or literature data [32,33]. In this study, for quality control purposes we developed and validated a simultaneous analysis method for luteolin-7-O-β-D-glucuronopyranosyl (1→2)-O-β-D-glucuronopyranoside, clerodendrin, chrysoeriol-7-O-diglucuronide, and diosmin (major components of L. chinensis extract) using HPLC-PDA (high-performance liquid chromatography performed using a photodiode array detector)
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