Simultaneous Determination of Four Active Ingredients in Sargentodoxa cuneata by HPLC Coupled with Evaporative Light Scattering Detection.

Di-Hua Li,Shu-Kun Zhang,Lei Yang,Yan Wang,Yuan-Shan Lv,Yu-Zhen Zhuo,Jun-Hong Liu

doi:10.1155/2016/8509858

Abstract

A HPLC coupled with evaporative light scattering detection method had been developed for the simultaneous determination of 3,4-dihydroxyphenylethyl alcohol glycoside, salidroside, chlorogenic acid, and liriodendrin in the stem of Sargentodoxa cuneata. With a C18 column, the analysis was performed using acetonitrile and 0.2% formic acid aqueous solution as mobile phase in gradient program at a flow rate of 0.9 mL/min. The optimum drift tube temperature of evaporative light scattering detection was at 105°C with the air flow rate of 2.5 L/min. The calibration curves showed good linearity during the test ranges. This method was validated for limits of detection and quantification, precision, and reproducibility. The recoveries were within the range of 96.39%–104.64%. The relative standard deviations of intraday and interday precision were less than 2.90% and 3.30%, respectively. The developed method can be successfully used to quantify the four analytes in the stem of Sargentodoxa cuneata from various regions in China.

Highlights

The stem of Sargentodoxa cuneata (Oliv.) Rehd. et Wils (Sargentodoxaceae) is a well-known herb of traditional Chinese medicine in China for treating abdominal pain due to acute appendicitis, sore and ulcer, dysmenorrheal, amenorrhea, traumatic swelling, and rheumatic arthritis [1]
The main active ingredients are believed to be salidroside, chlorogenic acid, and liriodendrin. These compounds are reported to possess effects such as antitumor [11], antiaging [12], antifatigue [13], hypolipidemic [14], antiviral [15], antiallergy [16], antioxidant [17], anti-inflammatory, analgesic [18], antimyocardial ischemia [9], antiarrhythmic [19], and calcium channel antagonistic [20]. Another phenolic glycoside such as 3,4-dihydroxyphenylethyl alcohol glycoside has been isolated from the stem of S. cuneata in recent years [21]
The standards of 3,4dihydroxyphenylethyl alcohol glycoside and liriodendrin [29] were isolated by the author from the stem of S. cuneata using preparative high performance liquid chromatography and resin

Summary

Introduction

The stem of Sargentodoxa cuneata (Oliv.) Rehd. et Wils (Sargentodoxaceae) is a well-known herb of traditional Chinese medicine in China for treating abdominal pain due to acute appendicitis, sore and ulcer, dysmenorrheal, amenorrhea, traumatic swelling, and rheumatic arthritis [1]. The main active ingredients are believed to be salidroside, chlorogenic acid, and liriodendrin These compounds are reported to possess effects such as antitumor [11], antiaging [12], antifatigue [13], hypolipidemic [14], antiviral [15], antiallergy [16], antioxidant [17], anti-inflammatory, analgesic [18], antimyocardial ischemia [9], antiarrhythmic [19], and calcium channel antagonistic [20]. An HPLC-ELSD method has been developed to simultaneously determine 3,4-dihydroxyphenylethyl alcohol glycoside, salidroside, chlorogenic acid, and liriodendrin in the stem of S. cuneata from various regions in China for the first time. Their contents in different samples of S. cuneata have been compared

Materials and Methods

Methods Validation

Method Validation

Discussion

Conclusion