Abstract
A method of high performance liquid chromatography-triple quadrupole mass spectrometry (HPLC-QqQ MS) in multiple reaction monitoring (MRM) mode for simultaneous determination of five Amadori compounds in tobacco has been developed. The separation was performed on an XBridge Amide column (250 mm x 4.6 mm, 3.5 microm) by gradient elution using methanol and water as mobile phases. The linearity of this method was good with the correlation coefficients (r) of 0.9895-0.9989. The limits of detection (S/N = 3) were from 3.51 microg/L to 14.86 microg/L, and the limits of quantitation (S/N = 10) were from 10.18 microg/L to 44.58 microg/L. The recoveries varied from 92.6% to 123.6% with the relative standard deviations (RSDs) less than 9.4%. The established method is rapid, accurate, robust and convenient, and it could be applied to the measurement of the five Amadori compounds in tobacco and cigarettes.
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