Simultaneous determination of electrophoretic and dielectrophoretic mobilities of human red blood cells

Abstract

Electrophoresis and dielectrophoresis of cells can reveal many distinct cellular properties but are often conducted separately. Herein a simultaneous strategy was proposed, and a simple method was established by making cells migrate through a cross channel under a micro video for real-time observation. The experiment can be performed within 0.044-1 s. In combination with digital calculation based on electromagnetic theory, the method was validated to be applicable to the determination of electrophoretic and dielectrophoretic mobilities, μEP and μDEP , of human blood erythrocytes, giving μEP = -(0.87 ± 0.16)× 10(-4) cm(2) ·V(-1) · s(-1) and μDEP = -(4.5 ± 1.3) × 10(-8) cm(4) ·V(-2) ·s(-1) by vector decomposition, or μEP = -(0.89 ± 0.14) × 10(-4) cm(2) ·V(-1) · s(-1) and μDEP = -(4.6 ±1.2) × 10(-8) cm(4) ·V(-2) · s(-1) by least squares fitting, all agreeing with published data. Hydrodynamic and EOFs were eliminated for better measurement. It was found that the location of cells had a serious impact on the measurement precision, and the upstream of the cross channel along the electric field was chosen for precise measurement. The method is also extendable to the study of other cells and particles.