Abstract
A ultra-high performance liquid chromatography-electrospray ionization tandem mass spectrometry (UHPLC-ESI-MS/MS) method was successfully developed and validated for the identification and determination of eight alkaloids: tetrahydropalmatine (A); palmatine (B); magnoflorine (C); columbamine (D); berberine (E); worenine (F); berberrubine (G) and coptisine (H) in rat plasma, which are the active components in Coptis deltoidea C. Y. cheng et Hsiao (CCY) and Coptis chinensis Franch (CF). The chromatographic separation of analytes was successfully achieved on an Agilent SB-C18 column (1.8 µm, 150 mm × 2.1 mm) using a programme with a mobile phase consisting of acetonitrile and water containing 0.3% acetic acid at a flow rate of 0.25 mL/min. The analytes were detected with a triple quadrupole tandem MS in multiple reaction monitoring (MRM) mode and an electrospray ionization (ESI) source in positive mode. The validated method showed good linearity over a wide concentration range (r2 > 0.991), and lower limits of quantification (LLOQ) less than 1.1 ng/mL for all analytes, and matrix effects ranged from 85.2% to 106.8%. The mean extraction recoveries were no less than 86.4%, and the precision and accuracy were within the acceptable limits. All analytes were proven to be stable during sample storage and analysis procedures. The method validation results demonstrated that the proposed method was sensitive, specific, and reliable, which could lay a foundation for the pharmacokinetic study of eight analytes after oral administration of CCY and CF in subsequent studies.
Highlights
Rhizoma Coptidis (RC), the dried rhizome of Coptis deltoidea C
chinensis Franch (CF) often referred to as ”Wei Lian”, has been more widely used as the major ingredient in many in kinds and content of alkaloid components exist between CCY and CF [8], which are caused by the Chinese herbal formulas such as oral liquid “shuang huanglian” [7]
The MS/MS ion-transitions were monitored in multiple reaction monitoring (MRM) mode to increase the specificity of the detection method to meet the requirements of the complexity of biological samples
Summary
Rhizoma Coptidis (RC), the dried rhizome of Coptis deltoidea C. CF often referred to as ”Wei Lian”, has been more widely used as the major ingredient in many in kinds and content of alkaloid components exist between CCY and CF [8], which are caused by the Chinese herbal formulas such as oral liquid “shuang huanglian” [7]. This plant is mainly produced differences their growth conditions, storage, and processing of the herb
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