Abstract
A specific, sensitive and rapid liquid chromatography tandem mass spectrometry (LC–MS/MS) method has been developed and validated for simultaneous quantification of doxorubicin and curcumin in rat plasma after intravenous administration. The analytes of doxorubicin and curcumin were extracted with methanol precipitation using glibenclamide as internal standard (IS). The chromatographic separation was performed on a C18 column with acetonitrile and 0.1% formic acid water as mobile phase and with gradient elution at a flow rate of 0.2mL/min. Calibration curves were linear over the ranges of 2–8000ng/mL for doxorubicin and 5–2000ng/mL for curcumin (r>0.99). The lower limit of quantification (LLOQ) was 2ng/mL for DOX and 5ng/mL for Cur. Finally, this developed method was successfully applied in the pharmacokinetic study of doxorubicin and curcumin in rats and evaluated the effects of curcumin on the absorption of doxorubicin after intravenous administration.
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