Abstract
A novel high-performance liquid chromatographic (HPLC) method with post-column detection was developed to simultaneously analyze the lipid hydroperoxides of polar and nonpolar lipids. The HPLC method uses a reversed-phase column (octyl-bonded silica) and a mobile phase of water/acetonitrile/methanol. Hydroperoxides were detected at 592 nm by using a ferrous (II)/xylenol orange (FeXO) reagent. This method enabled the separation of fatty acid hydroperoxides, phosphatidylcholine hydroperoxides, and hydroperoxides of neutral lipids, including triacylglycerol hydroperoxides and cholesterol ester hydroperoxides, by chromatography. These hydroperoxides could be quantified in a range between 40 pmol and 2 nmol. This new method was applied to estimate the lipid hydroperoxides formed during the photosensitized oxidation of rat plasma.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
