Abstract
Coumarins in Cham-dang-gwi, the dried root of Angelica gigas Nakai (AGN), possess pharmacological effects on anemia, pain, infection, and articular rheumatism. The AGN root containes decursin (D), decursinol angelate (DA), nodakenin, and decursinol (DOH), a major metabolite of D and DA. The aim of this study was to develop a simultaneous determination method for these four coumarins in human plasma using ultra high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). Chromatographic separation was performed on dual columns (Kinetex® C18 column and Capcell core C18 column) with mobile phase consisting of water and acetonitrile at a flow rate of 0.3 mL/min using gradient elution. Multiple reaction monitoring was operated in positive ion mode with precursors to product ion transition values of m/z 328.9→228.8, 328.9→228.9, 409.4→248.8, and 246.8→212.9 to measure D, DA, nodakenin, and DOH, respectively. Linear calibration curves were fitted over concentration range of 0.05–50 ng/mL for these four components, with correlation coefficient greater than 0.995. Inter- and intra-day accuracies were between 90.60% and 108.24%. These precisions were within 11.19% for all components. The established method was then applied to a pharmacokinetic study for the four coumarins after usual dosing in Korean subjects.
Highlights
The dried root of Angelica gigas Nakai (AGN) (Cham-dang-gwi in Korea) is a traditional medicine widely used to treat anemia, pain, infection, and articular rheumatism in Korea and other Asian countries [1,2]
Coumarins in AGN are composed of decursin (D), decursinol angelate (DA), nodakenin, (DA), nodakenin, and as decursinol (DOH) as of aD
The four coumarins were identified adopted because it had better sensitivity than negative mode
Summary
The dried root of Angelica gigas Nakai (AGN) (Cham-dang-gwi in Korea) is a traditional medicine widely used to treat anemia, pain, infection, and articular rheumatism in Korea and other Asian countries [1,2]. Coumarins have been found to be predominant constituents of Cham-dang-gwi. Coumarins in AGN are composed of decursin (D), decursinol angelate (DA), nodakenin,. (DA), nodakenin, and as decursinol (DOH) as of aD major DAactive 1).DAsand major components of active components of AGN, they possess pharmacological and biochemical activities both in vitro. AGN, they possess pharmacological and biochemical activities both in vitro and in vivo, including and in vivo,[3,4]. Including anti-cancer [3,4]
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