Simultaneous determination of contents of baicalin and ferulic acid in Qinmei granules by RP-HPLC method

Abstract

Objective: To establish a RP-HPLC method for simultaneous determination of contents of baicalin and ferulic acid in Qinmei granules.Methods: The separation was performed on a Platisial ODS C18 column(250 mm×4.6 mm,5 μm) with the mobile phase consisting of acetonitrile-0.085% phosphoric acid(24∶76)at a flow rate of 1.0 ml/min.The detection wavelength was 321 nm.Results: Baicalin and ferulic acid presented a good linearity in the range of 1.09-109.12 μg/ml(r=0.999 8) and 1.09~108.64 μg/ml(r=0.999 9),respectively.They had good intra-and inter-day precision,stability and reproducibility.Average recovery rates of low,middle and high concentrations of baicalin and ferulic acid in Qinmei granules were(99.69±1.24)%,(96.90±0.28)%,(95.06±0.44)%(n=3) and(95.90±1.13)%,(96.70±0.30)%,(102.52±1.18)%(n=3),respectively.Average contents of baicalin and ferulic acid in 3 batches of Qinmei granule samples were(5.85±0.016) mg/g(n=3) and(90±1.8) μg/g(n=3),respectively.Conclusion: It is an accurate,sensitive,reproducibility selective method for determination of the baicalin and ferulic acid in Qinmei granules.