Abstract
Purpose: To develop and validate a new, simple, sensitive and accurate high performance liquid chromatographic (HPLC) method for the simulataneous determination of matrine and secnidazole in suppositories.Methods: The method involved using a SinoChoom ODS-BP C18 column (5 ƒÊm, 4.6 mm ~ 200 mm) and mobile phase consisting of acetonitrile.triethylamine (0.05 %) in 0.025 mol/L KH2PO4 (20:80, v/v).The flow rate was 1 mL/min and detection was monitored at 210 and 311 nm for matrine and secnidazole, respectively. Total run time was 10 min and the column was maintained at 35 oC.Results: The excipients in the suppository did not interfere with the drug peaks. Matrine was eluted at a retention time (RT) of 4.30 min while linearity for the quantification of drug was obtained in the concentration range of 10.0 - 100.0 ƒÊg/mL (r2 = 0.9991). Secnidazole was eluted at a retention time (tr) of 6.69 min and linearity for the quantification of the drug was obtained in the concentration range of 10.0 - 150.0 ƒÊg/mL (r2 = 0.9993). Intra- and inter-day variations were < 1.0 % for both matrine andsecnidazole.Conclusion: The developed HPLC method was validated according to International Conference on Harmonisation (ICH) guidelines and proved to be suitable for the simultaneous determination of matrine and secnidazole in suppositories.Keywords: Matrine, Secnidazole, Suppository, HPLC, Assay
Highlights
Nature has been the repository of medicinal plants for thousands of years and a huge number of modern drugs have been isolated from natural sources, notably of plant origin [1]
E. guineensis extract showed in vitro antimicrobial activities against S. aureus with inhibition zone diameters of 14.0 mm
Further evidence of the alterations done to S. aureus by exposure to E. guineensis leaf extract is displayed in (Fig 2)
Summary
Nature has been the repository of medicinal plants for thousands of years and a huge number of modern drugs have been isolated from natural sources, notably of plant origin [1]. The African oil palm (Elaeis guineensis) is one of the plants that are central to the lives of traditional societies in West Africa. It is a monocotyledon perennial tree crop of the order Spodiciflorae and family Palmae,, grouped under the Cocoineae tribe. Staphylococcus aureus a Gram positive bacterium is responsible for numerous infections from skin to soft tissue infections It causes endocarditis and septic arthritis, which can induce bacteremia (associated with 80 % mortality in the preantibiotic era). On the other hand, permits the study of the surface of the organism These techniques were used to study the effect of E. guineensis leaf extract on the cell structure of S. aureus. The leaves were washed with water to remove dirt and dried in an oven at 40 °C for 7 days
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