Abstract
A simple and new simultaneous fourth derivative spectrophotometric method is proposed for the analysis of a two-component system containing cobalt(II) and nickel(II) without separation using 2-hydroxy-3-methoxy benzaldehyde thiosemicarbazone (HMBATSC) as a chromophoric reagent. The reagent reacts with cobalt(II) and nickel(II) at pH 6.0, forming soluble brown and yellow colored species, respectively. Cobalt(II) and nickel(II) present in themixture are simultaneously determined without solving the simultaneous equations bymeasuring the fourth derivative amplitudes at 468.5 nm and 474.5 nm, respectively. The derivative amplitudes obey Beer's law at 468.5 nm and 474.5 nm for Co(II) and Ni(II) in the range 0.059–3.299 μg mL−1 and 0.058–3.285 μg mL−1 respectively. A large number of foreign ions do not interfere in the present method. The present simultaneous method is used for the determination of micro amounts of cobalt in biological samples, nickel in plant samples, and in some alloy steels and soil sample.
Highlights
The direct spectrophotometric determination of metal ions in multicomponent systems is often complicated by interferences from the formulation matrix and spectral overlapping
Such interferences have been treated in many ways, such as solving two simultaneous equations [1] or using absorbance ratios at certain wavelengths [2,3,4]
Other approaches aimed at solving this problem have been used; including pH-induced differential spectrophotometry [6], least squares [7], and orthogonal function [5, 7, 8] methods
Summary
The direct spectrophotometric determination of metal ions in multicomponent systems is often complicated by interferences from the formulation matrix and spectral overlapping Such interferences have been treated in many ways, such as solving two simultaneous equations [1] or using absorbance ratios at certain wavelengths [2,3,4]. 2-hydroxy3-methoxy benzaldehyde thiosemicarbazone (HMBATSC) is used as a spectrophotometric reagent for simultaneous determination of cobalt(II) and nickel(II). Ni(II) complex with HMBATSC shows sufficient and measurable derivative amplitude at 474.5 nm and zero amplitude at 468.5 nm This difference in amplitudes was utilized to develop a fourth-order derivative spectrophotometric method for the simultaneous determination of cobalt(II) and nickel(II) in a mixture without solving simultaneous equations by measuring the fourth-order derivative amplitude at 468.5 nm and 474.5 nm, respectively
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