Abstract
A new simple and precise HPLC method has been developed for the estimation of chlorpheniramine maleate, dextromethorphan hydrobromide and pseudoephedrine hydrochloride in syrup pharmaceutical form. The separation of the mixture has been achieved in a short time. Chromatographic separation has been done on Chromegabond WR C18 column 5 μm 120 A 30 cm * 3.9 mm as stationary phase. The mobile phase was contained a 2 gm of sodium perchlorate in mixture of (200 mL acetonitrile + 500 mL methanol + 300 mL Water+ 2 ml triethylamine with a flow rate 1.5 ml/min. The PDA detection is 262 nm and the injection volume was 20 µl. The method has been validated for linearity with great correlation coefficients, accuracy recovery was 98.0-102., and precision with RSD less than 2.0%. This method was successfully used to separate and estimate these drugs purchased from Iraq. The analyzed syrup contains three active ingredients (chlorpheniramine maleate, dextromethorphan hydrobromide, pseudoephedrine hydrochloride) within 100 ±2 % of stated amount of the active ingredients. Validation of this method has been done and this method can be used for determination of these drugs in syrup pharmaceutical form.
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