Abstract
Secondary metabolites are an integral part of the plant kingdom which plays a vital role in development, growth, and defense. These are also very helpful for humans against different disorders. Phenols due to their strong antioxidant activity have a central role in conferring different types of biological activities. The present study aims at the simultaneous determination of chlorogenic acid and caffeic acid in the methanolic extracts of leaf samples of Siegesbeckia orientalis L. Simultaneous elution of both compounds has been attained by using toluene‒ethyl acetate‒formic acid‒methanol (6:6:1.6:0.6 v/v) as the mobile phase, and densitometric evaluation was done at 366 nm. The linear regression data for the calibration curve of both compounds show good linear relationship in the concentration range of 200–600 ng. The proposed method has been validated in terms of limit of detection, limit of quantification, specificity, precision, and accuracy studies. The present high-performance thin-layer chromatograp...
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