Simultaneous determination of Cefixime trihydrate and Ofloxacin in pharmaceutical dosage form by second order derivative UV spectrophotometry

Abstract

Derivative spectrophotometry offers a useful approach for the analysis of drugs in multi-component formulation. In this study a second order derivative spectrophotometric method is applied for the simultaneous determination of Cefixime Trihydrate and Ofloxacin in Tablet dosage form. The measurements were carried out at wavelengths of 307 and 298 nm for Cefixime Trihydrate and Ofloxacin respectively. The method was found to be linear (r=0.999) in the range of 4-20 ?g/ml for Cefixime Trihydrate in the presence of 20 ?g/ml of Ofloxacin at 307 nm. The linear correlation (r=0.999) was obtained in the range of 4-20 ?g/ml for Ofloxacin in the presence of 20 ?g/ml of Cefixime Trihydrate at 298 nm. The method was successfully used for simultaneous determination of Cefixime Trihydrate and Ofloxacin in tablet dosage form without any interference from excipients and prior separation.