Abstract
The simultaneous analysis of carbamazepine and its major metabolite 10, 11-carbamazepine epoxide in serum is described. A high-performance liquid chromatography (HPLC) system based on a μ-bondapack phenyl column (300 × 3·9 mm) and a UV detector (Λ = 214nm) were used. A mixture of methanol-water (50:50 v/v) at a flow rate of 1·5 mL min−1 was used as mobile phase. The proteins were precipitated with an acetonitrile solution containing benzophenone as an internal standard. Serum standard curves were linear over the range 0 to 20 μg mL−1 for carbamazepine and 0 to 5 μg mL−1 for carbamazepine epoxide. The limit of detection for both compounds was less than 10 ng mL−1. Analytical recoveries ranged from 80 to 85% for carbamazepine and 95 to 98% for carbamazepine epoxide. The method is a simple, sensitive and reproducible means of determining carbamazepine and its metabolite in serum.
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