Abstract
Abstract A rapid, accurate and precise method for the determination of buzepide, phenylpropanolamine, and clocinizine in a cough-mixture is described. The method is based on reversed-phase ion-pair HPLC. The only sample preparation necessary for the analysis is its dilution with the mobile phase. The resulting solution is analyzed on a column packed with Lichrosorb RP-18 (10μm) with acetonitrile in aqueous 5 10–2M sodium perchlorate, pH 3.0 (15:85) as initial mobile phase (2 mL·min-1) and detection at 220 and 254 nm. Then a linear gradient up to 95% acetonitrile in 20 min, is applied. The detection limits are 500 ng injected for phenylpropanolamine, 40 and 5 ng for buzepide and clocinizine, respectively.
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
