Abstract
Biogenic amines (BA) and estrogens (ES) have attracted increasing attentions due to their significance on food safety and food quality. In this study, a novel HPLC-FLD method using ethyl-acridine-sulfonyl chloride (EAC) as fluorescence labeling reagent has been developed for simultaneous determination of BA and ES in food samples. The labeling conditions including labeling time, pH of borate buffers and amount of EAC were optimized. The simultaneous labeling of BA and ES can be finished in as little as 6 min. This method without complex pre-treatment offered the low LOD of 0.27–0.69 ng/mL. It was applied to analyze several food samples (beer, cheese, fish, sausage and shrimp), and showed excellent applicability.
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