Simultaneous determination of aspartame, acesulfame-K, saccharin, citric acid and sodium benzoate in various food products using HPLC–CAD–UV/DAD

Małgorzata Grembecka,Zbigniew Fijałek,Agata Błażewicz,Piotr Baran,Piotr Szefer

doi:10.1007/s00217-013-2111-x

Małgorzata Grembecka, Zbigniew Fijałek + Show 3 more

Open Access

https://doi.org/10.1007/s00217-013-2111-x

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Abstract

A newly developed method for simultaneous determination of aspartame, acesulfame-K, saccharin, citric acid and sodium benzoate in various diet supplements and non-alcoholic beverages in a single run is presented. The analytes were analysed by high-performance liquid chromatography coupled to a charged aerosol (Corona CAD) and ultraviolet–diode array detectors simultaneously connected in series. Mass spectrometer MicrOTOF-QII from Bruker Daltonik (Bremen, Germany) was used to obtain the mass spectra for peak identifications. The method was validated using a Thermo Hypersil Gold-C18 column packed with 5 μm shell particles (150 × 4.6 mm) and methanol–water with 0.05 % TFA gradient mobile phase at a flow rate of 0.80 mL/min. The elaborated method was validated for linearity, precision and accuracy. The analytical results obtained in the validation study were highly satisfying; the recoveries for the analytes studied ranged between 98.1 and 101 % and the precision values from 0.11 to 1.73 %. By these procedures, the three sweeteners (aspartame, acesulfame-K and saccharin), citric acid and sodium benzoate could be well separated and quantitatively determined in varied food products. Hundred millilitres of soft drinks contained on average 5.50 mg of aspartame, 6.38 mg of saccharin, 8.94 mg of acesulfame-K, 9.05 mg of sodium benzoate and 111 mg of citric acid. Citric acid was the most abundant additive in all the samples analysed except for table sweeteners, and its highest concentration was determined in diet supplements, i.e. 347 mg/g. The percentage of adequate daily intake realisation in case of all additives is lower than 10 %, except for sodium benzoate in isotonic drinks (10.1 %).

Highlights

According to Community legislation food additive is defined as “any substance not normally consumed as a food in itself and not normally used as a characteristic ingredient of food whether or not it has nutritive value, the intentional addition of which to food for a technological purpose in the manufacture, processing, preparation, treatment, packaging, transport or storage of such food results, or may be reasonably expected to result, in it or its by-products becoming directly or indirectly a component of such foods” [1]
The chemicals used were of analytical-reagent grade: high-performance liquid chromatography (HPLC) grade methanol was obtained from J.T
Aspartame and acesulfame-K were obtained from Supelco (Bellefonte, USA), saccharin and citric acid from Aldrich Chemistry (Steinheim, Germany) while sodium benzoate from LGC Standards (Warsaw, Poland)

Summary

Introduction

According to Community legislation food additive is defined as “any substance not normally consumed as a food in itself and not normally used as a characteristic ingredient of food whether or not it has nutritive value, the intentional addition of which to food for a technological purpose in the manufacture, processing, preparation, treatment, packaging, transport or storage of such food results, or may be reasonably expected to result, in it or its by-products becoming directly or indirectly a component of such foods” [1]. Food additives may only be authorised if there is a technological need for their use; they do not mislead the consumer and they present no health hazard to the consumer. The Community legislation on food additives is based on the principle that only those additives that are explicitly authorised may be used. Most food additives may only be used in limited quantities in certain foodstuffs. If no quantitative limits are foreseen for the use of a food additive, it must be used according to good manufacturing practice, i.e. only as much as necessary to achieve the desired technological effect. Health effects and safety aspects of food are important issues for today’s consumers, and some people are concerned about safety of certain food additives such as sweeteners or preservatives [2].

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