Abstract
An immunoaffinity detection method for simultaneously determining the aflatoxins B1, B2, G1 and G2 in tobacco and tobacco products was developed via solvent extraction of sample, purifying andconcentrating on immunoaffinity column, pre-column derivatization by trifluoroacetic acid, separation byHPLC, and detection by fluorescence detector. The results showed that: 1) The determination could becompleted within 20 minutes, the four target aflatoxins were well separated and exhibited good linearrelations with correlation coefficients r 0.99. 2) The recoveries of the method ranged from 85% to 117% with the relative standard deviation(RSD) of 0.2%-9.4%(n=6), and the limits of detection andquantification of aflatoxin B1 were 0.10 and 0.34 μg/kg, respectively.
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