Simultaneous determination of adenine and guanine in DNA based on carboxylic acid functionalized graphene

Abstract

A nano-material carboxylic acid functionalized graphene (graphene − COOH) was prepared and used to construct a novel biosensor for the simultaneous detection of adenine and guanine. The direct electrooxidation behaviors of adenine and guanine on the graphene − COOH modified glassy carbon electrode were carefully investigated by cyclic voltammetry and differential pulse voltammetry. The results indicated that both adenine and guanine showed the increase of the oxidation peak currents with the negative shift of the oxidation peak potentials in contrast to that on the bare grassy carbon electrode. The electrochemical parameters of adenine and guanine on the modified electrode were calculated and a simple and reliable electroanalytical method was developed for the detection of adenine and guanine, respectively. The modified electrode exhibited good behaviors in the simultaneous detection of adenine and guanine with the peak separation as 0.334 V. The detection limit for individual determination of guanine and adenine was 5.0 × 10 −8 M and 2.5 × 10 −8 M (S/N =3), respectively. Furthermore, the measurements of thermally denatured single-stranded DNA were carried out and the value of (G + C)/(A + T) of single-stranded DNA was calculated as 0.80. The biosensor exhibited some advantages, such as simplicity, rapidity, high sensitivity, good reproducibility and long-term stability.