Abstract
A high-performance liquid chromatographic assay for the simultaneous determination of abscisic acid and jasmonic acid from plant extracts is described. The method is based on the derivatization of the plant growth regulators with a fluorescent hydrazide to give stable fluorescent products. The use of fluorescent derivatives allowed detection of abscisic acid and jasmonic acid at 5 pmol and provided a linear response to 15 nmol. The method is selective and reproducible, and yields single peaks for each compound regardless of isomer. The identity of the dansyl hydrazone plant growth regulator peaks was supported by chromatography of the N-dimethylanthranilohydrazones of the compounds. The use of two fluorescent hydrazides makes routine confirmation of peak identity a simple matter. The presence of abscisic acid and jasmonic acid in tissue extracts was also supported by chromatographic identification of corresponding methyl and ethyl esters following esterification. Chromatographic peaks corresponding to the dansyl hydrazones of abscisic and jasmonic acid were obtained from extracts of several different tissues of soybean [ Glycine max (L.) Merr.], snap beans ( Phaseolus vulgaris L.), lima beans ( Phaseolus lunatus L.), and broccoli ( Brassica oleracea L.).
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