Simultaneous Determination of 19 Antibiotics and 19 Anthelmintics Residues in Edible Fungi by UHPLC-MS/MS in Combination with QuEChERS Method

Abstract

A novel sensitive method for simultaneous determination of 19 antibiotics and 19 anthelmintics in edible fungi using QuEChERS-UHPLC-MS/MS method was established. For every 10.0 g sample, 20 mL of acetonitrile with 2% acetic acid and 1.2 g sodium acetate were used for extraction, followed by 6.0 g anhydrous MgSO4 for dehydration, and 50 mg anhydrous MgSO4+10 mg PSA for purification. The optimized QuEChERS method presented excellent recoveries ranging from 85.31% to 103.94%, with relative standard deviations of 3.65–18.31%. Limit of detection and limit of quantification of the analysis method were 0.24–19 ng/L and 0.8–65 ng/L, respectively. The method was applied to analyze the 38 target compounds in 8 kinds of edible fungi samples from markets in Chengdu, China. The results indicated that the antibiotics and anthelmintics were widely existed with concentrations ranging from below LOD to 296 μg·L−1. The concentrations of sulfamethoxazole, sulfadiazine, thiabendazole and fenbendazole were relatively high. Based on the current study, exposure to antibiotics and anthelmintics through edible fungi consumption is harmless to human. However, long term exposure to low doses of antibiotics and anthelmintics still needs attention and merits further study.