Abstract
Psidium guajava, a popular food and medicine dual purposes plant cultivated in tropical and subtropical regions, has been widely used as food crop and folk medicine, such as anti-diabetes agent, around the world. Triterpenoids have been considered as the major active ingredients of P. guajava. In the present study, a high-performance liquid chromatography coupled with diode array and evaporative light scattering detectors (HPLC–DAD–ELSD) method was developed for simultaneous determination of nine triterpenoids in P. guajava. Pressurized liquid extraction (PLE) was performed for sample preparation, and the analysis was achieved on a Cosmosil 5C18-MS-II (Nacalai Tesque, Kyoto, Japan) column eluted with gradient 0.1% aqueous formic acid-methanol system. The drift tube temperature of ELSD was set at 40 °C, and nitrogen flow-rate was at 1.6 L/min. All calibration curves for the analytes showed good linear regression (R2 > 0.9992) within test ranges. The established method was validated for intra-day and inter-day precisions (RSDs < 5%) and accuracy (recovery 94.23–106.87%). The validated method was successfully applied to determinate nine triterpenoids in 15 samples from the leave or fruit of P. guajava. In addition, the α-glucosidase inhibition assay showed good α-glucosidase inhibition activity in almost all the determined triterpenoids. The present study suggested that triterpenoids should be the quality control markers for P. guajava and HPLC–DAD–ELSD was an effective tool for the quality control of P. guajava.
Highlights
Psidium guajava, an important Myrtaceae family plant cultivated in tropical and subtropical regions, is widely used as food crop and folk medicine around the world [1]
The optimization of High-Performance Liquid Chromatography (HPLC) conditions was carried out using sample PGL-2 (P. guajava leaves from Qingping)
The developed HPLC–DAD–ELSD method was further validated for linearity, limit of detection (LOD), limit of quantification (LOQ), interThe developed HPLC–DAD–ELSD method was further validated for linearity, LOD, LOQ, and intra-day precision and accuracy
Summary
An important Myrtaceae family plant cultivated in tropical and subtropical regions, is widely used as food crop and folk medicine around the world [1]. 3β-O-cis-p-coumaroyl-2α-hydroxy-urs-12-en-28-oic acid (5), activities of P. guajava [19,20,21,22,23,24]. Our previous study showed acid (jacoumaric acid) (7) were identified from P. guajava; esters of maslinic acid or that these compounds could be transformed into maslinic acid and corosolic acid by hydrochloric corosolic acid [25]. HPLC–PDA method was established for quantification of ursolic acid and guajava [29]. An HPLC-DAD-ELSD and pressurized liquid extraction developed to simultaneously determine nine triterpenoids The inhibitive effect of its leaves and fruits of guajava collected from different locations. The inhibitive effect of its leaves and fruits, as well as nine analytes on α-glucosidase were examined and compared.
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