Simultaneous Determination and Pharmacokinetic Study of Quercetin, Luteolin, and Apigenin in Rat Plasma after Oral Administration of Matricaria chamomilla L. Extract by HPLC-UV.

Xiaoxv Dong,Jian Ni,Chunjing Yang,Wei Lan,Xingbin Yin,Wenping Wang

doi:10.1155/2017/8370584

Abstract

A simple and sensitive HPLC-UV method has been developed for the simultaneous determination of quercetin, luteolin, and apigenin in rat plasma after oral administration of Matricaria chamomilla L. extract. The flow rate was set at 1.0 ml/min and the detection wavelength was kept at 350 nm. The calibration curves were linear in the range of 0.11–11.36 μg/ml for quercetin, 0.11–11.20 μg/ml for luteolin, and 0.11–10.60 μg/ml for apigenin, respectively. The intraday and interday precisions (RSD) were less than 8.32 and 8.81%, respectively. The lower limits of quantification (LLOQ) of the three compounds were 0.11 μg/ml. The mean recoveries for quercetin, luteolin, and apigenin were 99.11, 95.62, and 95.21%, respectively. Stability studies demonstrated that the three compounds were stable in the preparation and analytical process. The maximum plasma concentration (Cmax) was 0.29 ± 0.06, 3.04 ± 0.60, and 0.42 ± 0.10 μg/ml, respectively. The time to reach the maximum plasma concentration (Tmax) was 0.79 ± 0.25, 0.42 ± 0.09, and 0.51 ± 0.13 h, respectively. The validated method was successfully applied to investigate the pharmacokinetics study of quercetin, luteolin, and apigenin in rat plasma after oral administration of M. chamomilla extract.

Highlights

Matricaria chamomilla L. (German chamomile), a member of the Asteraceae family, is native to southern and eastern Europe and cultivated in countries of America and Asia [1,2,3]
An appropriate IS will control variability in extraction and HPLC injection. Several substances, such as chloromycetin, naringenin, genkwanin, and kaempferol were tested as internal standards
Its retention time was suitable and it was well separated from quercetin, luteolin, and apigenin

Summary

Introduction

Matricaria chamomilla L. (German chamomile), a member of the Asteraceae family, is native to southern and eastern Europe and cultivated in countries of America and Asia [1,2,3]. A diverse range of pharmacological properties have been demonstrated for this plant including anti-inflammatory [6, 7], antimicrobial [8], anticancer [9], analgesic [10], antipruritic [11], antiulcer [12], and acaricidal [13]. Previous phytochemical investigations on M. chamomilla have led to the isolation of essential oil, flavonoids, terpenoids, and coumarins [15,16,17,18,19]. Among these chemical compositions, flavonoids are the main active constituents present in the plant, which exhibited anxiolytic and antidepressant activity [20, 21]. To our knowledge, there has been no report on the simultaneous determination of quercetin, luteolin, and apigenin in animal plasma after oral administration of M. chamomilla extracts

Methods

Results

Discussion

Conclusion