Abstract
Enterohaemorrhagic Escherichia coli (EHEC), mainly the serotype O157:H7 is a global concern in foodborne outbreaks,. The incidence of E. coli O157:H7 in seafood sold in India has been reported as 8.4%. The conventional method for the detection of E. coli O157:H7 from seafood is cumbersome and time consuming making this strain particularly challenging to detect. In this study a multiplex PCR was developed targeting six major virulence factors of the pathogen viz. shigatoxin 1 and 2, attachment and effacement factor, flagellar antigen, hemolysin gene and O157 antigen. The sensitivity of the multiplex PCR developed in the study was found to be 120 pg μl-1 of DNA and that of E. coli 0157:H7 spiked samples was 2 cfu ml-1. The multiplex PCR developed in this study showed 100% specificity in identifying E. coli O157:H7, effectively distinguishing it from non-target bacteria. This highly sensitive and specific multiplex PCR is the first of its kind to detect E. coli O157:H7 in seafood by targeting six major virulence genes. Keywords: E. coli O157:H7, EHEC, Foodborne pathogen, Seafood, Sensitivity, Shigatoxin, Specificity
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
