Abstract
Ruthenium hexacyanoferrate/reduced graphene oxide (RuHCF/rGO) electrode was fabricated by electrochemical deposition of RuHCF on a rGO-modified electrode. Electrocatalytic oxidation and determination of glutathione, threonine, and glycine were carried out simultaneously using the modified electrode. The electrochemical sensor showed a wide linear response in the concentration range of 5.12–25.58 μM for glutathione, 1.98–15.86 μM for threonine, and 1.25–7.49 μM for glycine. The RuHCF/rGO–modified electrode showed minimal interference in the presence of related compounds. The limit of detection observed for glutathione, threonine, and glycine was 1.70, 0.66, and 0.4 μM respectively. Saliva samples were used for checking the analytical utility of the RuHCF/rGO–modified electrode for sensing the three analytes, and the recovery percentages were between 95.80 and 98.03. The electrode also exhibited long-term stability and shelf life.
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