Abstract
This paper introduces a method for simultaneous detection of 2019 novel coronavirus (2019-nCoV) and influenza virus A and influenza virus B (FluA/B) by dual fluorescent RT-PCR, providing some references for the current clinical first-line practice against the epidemic. More than 12000 samples of nasopharyngeal swabs, sputum and anal swabs were collected. All samples were inactivated at 60°C for 40 min before testing, so as to protect medical examine. Nucleic acid detection kits from two manufacturers of 2019-nCoV and FluA/B were selected for the detection. Carboxyfluorescein (FAM) and green fluorescent protein (VIC) labeled probes were used to achieve simultaneous detection of the four gene targets using a double fluorescent RT-PCR reaction system. According to the results of nucleic acid detection, there is no cross infection between 2019-nCoV and FluA/B. The CT value of novel coronavirus nucleic acid in anal swab greater than CT value of sputum greater than CT value of nasopharyngeal swab in the same patient. This paper introduces a method for rapid and simultaneous detection of 2019-nCoV and FluA/B by dual fluorescent RT-PCR. It was improved the detection efficiency and reduce the cost, which could be used for rapid and emergent detection of 2019-nCoV and FluA/B. Our experiment is fast and accurate, could make reference for nucleic acid detection medical diagnosis for clinical medical staff.
Highlights
This paper introduces a method for simultaneous detection of 2019 novel coronavirus (2019-nCoV) and influenza virus A and influenza virus B (FluA/B) by dual fluorescent RT-PCR, providing some references for the current clinical first-line practice against the epidemic
"COVID-19", referred to as Corona Virus Disease 2019, is a pneumonia caused by infection of a novel coronavirus. [1,2]
The novel coronavirus continues to spread around the world, with confirmed cases on six continents except for Antarctica
Summary
"COVID-19", referred to as Corona Virus Disease 2019, is a pneumonia caused by infection of a novel coronavirus. [1,2]. "COVID-19", referred to as Corona Virus Disease 2019, is a pneumonia caused by infection of a novel coronavirus. Since December 2019, some hospitals in Wuhan, Hubei Province, have reported a number of pneumonia cases for unknown reasons due to exposure to the South China Seafood Market. There have been more than 80,000 people infected by 2019 Novel Coronavirus in China, among whom more than 3,000 people died. Besides China, the epidemic situation in Italy, America, Spain, Germany, and Iran is worsening rapidly. Quick and accurate diagnosis on infection of the novel coronavirus is very important for assessment and control of COVID-19 situation in the world. This paper is mainly to introduce simultaneous detection of novel coronavirus and FluA/B by dual fluorescence RT-PCR method
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