Abstract
We investigated the frequency of H274Y-positive swine-origin 2009 A (H1N1) influenza virus outbreak in Thailand during May-August 2009. This study sought to find Oseltamivir resistance mutation H274Y by using pyrosequencing. From 8,710 real-time RT-PCR swine-origin 2009 A(H1N1) influenza virus-positive specimens, 100 randomly selected samples identified one such virus with H274Y mutation using pyrosequencing. The patient probably acquired oseltamivir resistance from natural variation, since he had never received that form of treatment before and recovered from influenza-like symptoms without using anti-influenza drugs.
Highlights
We investigated the frequency of H274Y-positive swine-origin 2009 A (H1N1) influenza virus outbreak in Thailand during May-August 2009
Of the 8,710 swine-origin 2009 A (H1N1) influenza virus-purified RNA positive samples, 100 samples were randomly selected for pyrosequencing
One of the samples was identified with H274Y mutation by pyrosequencing
Summary
We investigated the frequency of H274Y-positive swine-origin 2009 A (H1N1) influenza virus outbreak in Thailand during May-August 2009. Methodology: This study sought to find Oseltamivir resistance mutation H274Y by using pyrosequencing. Results: From 8,710 real-time RT-PCR swine-origin 2009 A(H1N1) influenza virus-positive specimens, 100 randomly selected samples identified one such virus with H274Y mutation using pyrosequencing. Conclusions: The patient probably acquired oseltamivir resistance from natural variation, since he had never received that form of treatment before and recovered from influenza-like symptoms without using anti-influenza drugs. The primary treatments for influenza A infection are the neuraminidase inhibitors (NAI) oseltamivir and zanamivir. They inhibit virus replication by mimicking the natural substrate of the influenza neuraminidase (the sialic acid receptors), and bind to the active site, preventing neuraminidase from cleaving host-cell receptors or releasing new viruses [1]. A low prevalence of NAI resistance has been detected among human influenza viruses circulating worldwide [4]
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