Abstract
Although this study aims to develop an improved method for the preservation of reserve lipids in plant tissues for different uses in plant anatomy, it mostly aims to develop an improved method for the identification of lipid reserves where synthesis or storage occurs. The proposed procedures entail only the utilization of (2-hydroxyethyl)-methacrylate (HEMA) as a dehydration agent. One of the procedures is based on the gradual exchange of aqueous HEMA solutions with increasing concentrations. In another procedure, dehydration and infiltration are induced by the presence of silica gel around a modified microcentrifuge tube containing the aqueous HEMA solution with the plant tissues, thus allowing efficient lipid preservation. Both procedures resulted in simultaneous dehydration and infiltration of the endosperm and embryo of Ricinus communis, while eliminating the use of ethyl alcohol, thus providing better lipid preservation.
Highlights
Among the different histological procedures in plants, the most widely used procedure involves chemical fixation, dehydration, infiltration, and embedding of cells and tissues
ABSTRACT this study aims to develop an improved method for the preservation of reserve lipids in plant tissues for different uses in plant anatomy, it mostly aims to develop an improved method for the identification of lipid reserves where synthesis or storage occurs
Dehydration and infiltration are induced by the presence of silica gel around a modified microcentrifuge tube containing the aqueous HEMA solution with the plant tissues, allowing efficient lipid preservation
Summary
Among the different histological procedures in plants, the most widely used procedure involves chemical fixation, dehydration, infiltration, and embedding of cells and tissues. The loss of proteins, carbohydrates, and lipids is common during these steps (Hayat 1970; O’Brien & McCully 1981; Gerrits et al 1990; Gerrits et al 1992) Such losses lead to difficulties in structural, physiological, and functional interpretation. In this context, unfixed cellular elements are considered to be removed by solvents, there have been reports in the literature of loss of neutral lipids and phospholipids even after fixation by osmium tetroxide (Korn & Weisman 1966). Sugars and lipids are lost during the processes of dehydration by ethanol or organic solvents as well as during infiltration and embedding (Baker 1958; Korn & Weisman 1966; Hayat 1970)
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