Simultaneous Confocal based 3D Tracking and Fluorescence Imaging

Abstract

The response of the receptor, IgE-FceRI, to binding of a multivalent antigen begins a cellular signaling cascade resulting in the release of histamine, part of the human allergic response. The receptor's modes of motion and internalization process are investigated using a confocal based 3D tracking microscope. A Nipkow spinning disk has been added to the microscope to allow simultaneous confocal 3D tracking and wide field spinning disk imaging. The receptor is tracked in live cells while simultaneously imaging cellular structures labeled with fluorescent proteins.