Abstract
Simultaneous biochemical reaction and separation has been carried out successfully for the first time in a continuous rotating annular chromatograph (CRAC) by inverting sucrose to glucose and fructose using the enzyme invertase. The chromatograph was packed with 14.5 dm 3 Dowex 50W-X4 calcium form ion exchange resin. Results from the initial experiments indicated that complete conversion could be achieved for feed concentrations of up to 50% w/v sucrose and at feed throughputs of up to 15 kg sucrose per m 3 resin/h. Numerical simulation for the combined biochemical and separation on a CRAC has also been carried out. The model was solved using a finite difference method and the results indicate a good agreement between the experimental and the predicted elution concentration profile.
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