Abstract
A hydrothermal–chemical process for the simultaneous biomass hydrolysis and bio-hydrogen formation was proposed in this study. Current results revealed that a maximum reducing sugar concentration of 26.01 g/L and the yield of bio-hydrogen of 0.9098 mmol/g-cellulose were attained with the designated experimental parameters. Consequently, this study provided a potential route to produce bio-hydrogen and reducing sugar simultaneously in a one-step reaction process by using the energy crops or the agriculture wastes as the substrate.
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