Abstract
PurposeTo demonstrate the feasibility of imaging a bolus of co‐polarized [1‐13C]pyruvate and 13C‐urea to simultaneously assess both metabolism and perfusion in the rodent heart.MethodsCopolarized [1‐13C]pyruvate and 13C‐urea was imaged using a multi‐echo, flow‐sensitized spiral pulse sequence. Healthy rats were scanned in a two‐factor factorial design (n = 12 total; metabolism: overnight fasting versus fed with dichloroacetate injection; perfusion: rest versus adenosine stress‐induced hyperemia).ResultsAlterations in metabolism were detected by changes in pyruvate metabolism into 13C‐bicarbonate. Statistically independent alterations in perfusion were detected by changes in myocardial pyruvate and urea signals.ConclusionThe new pulse sequence was used to obtain maps of metabolism and perfusion in the rodent heart in a single acquisition. This hyperpolarized 13C imaging test is expected to enable new studies in which the cardiac metabolism/perfusion mismatch can be studied in the acute environment. Magn Reson Med 77:151–158, 2017. © 2016 The Authors Magnetic Resonance in Medicine published by Wiley Periodicals, Inc. on behalf of International Society for Magnetic Resonance in Medicine
Highlights
Following acute myocardial infarction, the assessment of tissue viability and the presence of the salvageable “area respect to concentration [8,9]
We investigated the feasibility of using an injection of copolarized [1-13C]pyruvate and 13C-urea combined with a flow-sensitized spiral multiecho pulse sequence to simultaneously assess both myocardial metabolism and perfusion in a single acquisition
The flow-sensitized spectrum showed reduced amplitude pyruvate (15%) and urea resonances (20% of the non– flow-suppressed amplitude). 13C-bicarbonate, which is produced in the mitochondria via pyruvate dehydrogenase (PDH), is largely intracellular, and remained similar in amplitude in both spectra (80% of the non–flow-suppressed amplitude)
Summary
The assessment of tissue viability and the presence of the salvageable “area respect to concentration [8,9] These two hyperpolarized 13C measurements of tissue metabolism and perfusion have great potential in assessing tissue viability and the presence of hibernating myocardium. Simultaneous hyperpolarization (ie, copolarization) of multiple 13C-labeled substrates has been reported and offers an attractive method to obtain multiple readouts of both enzymatic and physiological properties in vivo in a single imaging experiment [10,11,12]. This approach has not been explored in the context of cardiac imaging.
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