Abstract

A simple and reliable technique for the simultaneous estimation of serum triiodothyronine (T3) and thyroxine (T4) is discussed. T3 assay was done by the solid phase technique using antibody coated Eppendorf pipette tips. T4 assay was done by the polyethylene glycol separation system. The assay used 50 μl of serum sample. Inter-assay and intra-assay coefficient of variation are less than 12% throughout the assay range, for both assays.

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