Simultaneous Application of Nanomaterials to Separation of Phosphorylated and Glycosylated Proteins

Abstract

Glycosylation and phosphorylation are two ubiquitous and significant post-translational modifications (PTMs), which have been described in the previous chapters. With the advancement of research on glycoproteomics and phosphoproteomics, it is revealed that glycosylation and phosphorylation not only play individually important roles in in vivo, but also regulate some functions of proteins through interaction in most cases. As a consequence, the simultaneous analysis of glycosylation and phosphorylation attracts much attention to reveal their relations and understand some biological mechanisms. Mass spectrometry (MS) is the primary tool to analyse and detect PTM proteins. For the problems such as wide variety of proteins and the low ionization efficiency of glycosylation and phosphorylation during MS analysis, the efficient techniques based on nanomaterials for the simultaneous enrichment of glycosylated and phosphorylated proteins from complex samples are developed in recent years. This chapter will introduce the preparation and application of these functional nanomaterials in the simultaneous analysis of glycosylation and phosphorylation.