Abstract
BackgroundBrdU is a commonly used reagent in cell proliferation assays, and WST-1 measurement is widely used to detect cell viability. However, no previous study has formally reported the combination of the two assays, which may be used to detect the proliferation and viability simultaneously. In this study, we examined the effect of adding BrdU 2 h prior to the WST-1 assay and tried to test the possibility of the combined detection using rat airway smooth muscle cells.ResultsThe WST-1 measurements obtained from the combined detection were consistent with those obtained from the separate detection, which suggested that the addition of BrdU 2 h prior to the WST-1 analysis did not affect the WST-1 results. The BrdU measurements obtained from the combined detection also demonstrated the same trend as that obtained from the separate detection, and dosages of 200, 400 and 800 ng/ml testing reagent significantly inhibited the proliferation of rat airway smooth muscle cells.ConclusionsOur study suggests that the BrdU and WST-1 measurements can be applied simultaneously without mutual interference, which may increase the efficacy and consistency of these measurements to a certain extent.
Highlights
BrdU is a commonly used reagent in cell proliferation assays, and WST-1 measurement is widely used to detect cell viability
The expression levels of BrdU (Bromodeoxyuridine), Ki-67, and PCNA have been compared by immunohistochemical labeling to evaluate the proliferation of epidermal basal cells, and the results showed that there was no substantial difference among the three methods [5]
We examined the effect of adding BrdU 2 h prior to the WST-1 assay and tried to test the possibility of the combined detection
Summary
BrdU is a commonly used reagent in cell proliferation assays, and WST-1 measurement is widely used to detect cell viability. Many methods have been established for cell number quantification, and all of these have underlying assumptions, relative merits, and must be applied appropriately. Both direct and indirect techniques have been used for measuring cell proliferation. The indirect measurement methods included isotope-related methods and non-radioactive assays. Isotopes, such as 3H, 76Br, 15 N and 13C, are used to detect cell proliferation, but radioactive products and/or special facilities are required.
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