Simultaneous and rapid determination of lomefloxacin and chlortetracycline hydrochloride residues in meat foods by Al3+ sensitized synchronous fluorescence spectrometry

Abstract

The fluorescence intensity of lomefloxacin and chlortetracycline hydrochloride were significantly enhanced after the complexation of the two antibiotics with Al3+ and the synchronous fluorescence spectra of the two complexes could be well separated when Δλ = 70 nm. Based on the above facts, a new method combining Al3+ sensitization with synchronous fluorescence spectrometry was developed to determine lomefloxacin and chlortetracycline hydrochloride residues in meat foods in this study. This study showed that the concentration of the two antibiotics had an excellent linear relationship with the synchronous fluorescence intensity in the range of 1–550 ng mL−1 and 5–1800 ng mL−1. Detection limits of the two antibiotics were 0.0378 μg kg−1 and 0.3530 μg kg−1, respectively. Recovery rates for lomefloxacin and chlortetracycline hydrochloride were 86.1 - 90.2% and 85.3 - 96.5% in meat samples, respectively. The novel synchronous fluorescence spectrometry established in the experiment had the advantages of high sensitivity, good selectivity, low detection limit, fast detection speed and high accuracy. It had been applied to the detection of lomefloxacin and chlortetracycline hydrochloride residues in meat with satisfactory results.