Abstract
An Isothermal gas chromatographic method for the analysis of the free-acid forms of the antiepiliptic drugs (AED's) has been developed. A single-step extraction requiring 0.25 to 0.50 ml of serum and 5 ml of methylene chloride is used. Serum Iipids and certain vacutainer plastlcizers are virtually eliminated by performing the extraction in the presence of ammonium sulfate at pH %2, resulting In a remarkably clean extract. The column consists of a mixed stationary phase specifically developed as a cooperative effort of the South Bend Medical Foundation Department of Toxicology and Supelco, Inc. for separation of phenobarbital, carbamazepine, primidone, phenytoin and one or more of their appropriate internal standards. Analysis of ethosuximide and other less common AED's can also be performed by employIng suitable internal standards and oven temperatures. Analysis of a single patient sample can be completed in 20 minutes including extraction, concentration, and chromatographic analysis.
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