Simultaneous Analysis of Topotecan and Capsaicin by Micellar Liquid Chromatography

Abstract

Objectives: This study aimed to develop a micellar liquid chromatography-based simultaneous estimation method for the analysis of topotecan and capsaicin. Materials and Methods: The Liquid chromatographic system consisted of the Shimadzu Prominence HPLC model (Shimadzu Corp., Kyoto, Japan) containing an LC-20AT isocratic pump, an autosampler SIL-20AC, and a diode array detector SPD-M20 A (190–800 nm) was used. The drugs were analyzed at a wavelength of 230 nm using sodium dodecyl sulfate, buffer salt (0.01 M sodium dihydrogen phosphate), and n-butanol (7%) as mobile phase. Results: The developed Micellar liquid chromatography-Photodiode Array Detection (MLC-PDA) method successfully eluted the topotecan and capsaicin with retention times of 6.2 min and 16.3, respectively. The developed method has displayed the limits of detection i.e. 0.05 and 0.98 μg/mL and limits of quantification i.e 0.08 and 1.25 μg/mL for topotecan and capsaicin, respectively. Statistical analysis further demonstrated that the developed method is linear, exact, accurate, and specific for the analysis of topotecan and capsaicin. The method was verified as per the ICH guidelines. Conclusion: The developed MLC-PDA method was found to be precise, simple, and reproducible. The developed MLC-PDA method successfully estimated the topotecan and capsaicin from the combination of drugs. Micellar chromatography is an accurate, rapid, cost-effective, and less hazardous method as compared to HPLC.