Abstract
Tannin, condensed and hydrolysable, and lignin are two of the most abundant natural biopolymers in the environment. Yet it is still not possible using one analytical technique to simultaneously track each of their dynamics in litter and soils. Thermally assisted hydrolysis and methylation (THM) using tetramethylammonium hydroxide (TMAH) has been employed to investigate the cycling of tannin and lignin forms in one analysis, but sometimes with equivocal results as many of the products released may originate from both biopolymers. In this study we applied 13C-labeled TMAH in the analysis of soil organic matter in a soil profile under Corsican pine, an input lacking in hydrolysable tannins, to show the potential of this tool in tracking complex phenol chemistry. This method successfully allowed for a qualitative distinction among tannin and lignin input as well as permitting the inference of ring specific decay of condensed tannins.
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